Cloning and partial characterization of a gene in Bombyx mori homologous to a human adiponectin receptor.

Cloning and Characterization of NAD-dependent Deacetylase Sirtuin 2 Homolog from the Silkworm, Bombyx mori

Characterization and Prokaryotic expression of Glucuronyltransferase-S Gene in Silkworm Bombyx mori

As genome of B. mori is available in GenBank, identification of novel genes of B. mori can be carried out. In this study, we used the in silico cloning method to obtain the Glucuronyltransferase-S (GlcAT-S) gene of B. mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR,prokaryotic expression and western blot. The GlcAT-S cDNA contains a 843bp ORF and has three exons....

in vitro Anti-Proliferative Effect of Adiponectin on Human Endometriotic Stromal Cells through AdipoR1 and AdipoR2 Gene Receptor Expression

Background: Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (ESCs) p...

Assessing Genetic Diversity in Iranian Native Silkworm (Bombyx mori L.) Strains and Japanese Commercial Lines Using AFLP Markers

Three Iranian native strains and three Japanese commercial lines of the silkworm Bombyx mori were analyzed using amplified fragment length polymorphism (AFLP) markers. A set of ten PstI/TaqI primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. Estimates of Nei’s gene diversity for all loci in individual strains and commercial lines indicated a higher degre...

Cloning and characterization of a mariner-like element in the silkworm, Bombyx mori.

We have identified a mariner-like element (MLE) in the genome of the silkworm, Bombyx mori. The cloned MLE, desiqnated as BmMLE, contained an ORF that was interrupted by insertions, stop codons, and frameshifts. The complete ORF sequence was estimated by sequencing the PCR products. The hypothetical protein coded by this ORF showed 34% homology to the Mos1 element of Drosophila mauritiana, and ...